Journal: Cell death discovery

Article Title: CD5L deficiency attenuate acetaminophen-induced liver damage in mice via regulation of JNK and ERK signaling pathway.

doi: 10.1038/s41420-021-00742-3

Figure Lengend Snippet: Fig. 6 The proliferation of hepatocytes is increased in CD5L−/−mice. A Immunohistochemical staining of PCNA in WT and CD5L−/−mouse liver at the indicated time point after PBS or APAP treatment (n = 5 for each group at each time point). Deeply stained nucleus indicates positive results. The ratio of PCNA positive cells to total hepatocytes was calculated. Representative images are shown. Scale: 100 μm. B Vascular endothelial permeability after APAP treatment for 24 h was determined by Evans Blue assay (n = 8 for each group). The results are presented as means ± SD of at least three independent experiments. *P < .05; **P < 0.01.

Article Snippet: Immunohistochemical (IHC) staining was performed using CD5L antibody (#50020-T24, Sino Biological, Beijing, China), PCNA antibody (#13110, Cell Signaling Technology, Beverly, MA, USA) and sheep anti-APAP polyclonal Fig. 6 The proliferation of hepatocytes is increased in CD5L−/− mice.

Techniques: Immunohistochemical staining, Staining, Permeability, Evans Blue Assay